4AH3
Crystal structure of the holo omega-transaminase from Chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 61.890, 62.230, 119.610 |
Unit cell angles | 75.14, 81.73, 76.17 |
Refinement procedure
Resolution | 115.190 - 1.570 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.27322 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qj3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.459 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.730 | 1.660 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.100 | 0.300 |
Number of reflections | 218828 | |
<I/σ(I)> | 4.4 | 1.9 |
Completeness [%] | 94.4 | 90.9 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |