4AH1
CRYSTAL STRUCTURE OF THE DB 921-D(CGCAAATTTGCG)2 COMPLEX AT 1.42 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.622, 38.881, 62.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.040 - 1.420 |
| R-factor | 0.18718 |
| Rwork | 0.184 |
| R-free | 0.25036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2r |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.408 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 1.500 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.050 | 0.470 |
| Number of reflections | 11003 | |
| <I/σ(I)> | 13.8 | 2.8 |
| Completeness [%] | 96.6 | 98.2 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | MAGNESIUM CHLORIDE, DNA, DB921, MPD, SPERMINE TETRAHYDROCHLORIDE, VAPOUR DIFFUSION, SITTING DROP, TEMPERATURE 285 K, PH 6.50 |
