4AGV
Structure of a tetrameric galectin from Cinachyrella sp. (Ball sponge)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2010-02-21 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 38.960, 66.510, 71.550 |
Unit cell angles | 117.07, 95.39, 99.56 |
Refinement procedure
Resolution | 35.610 - 2.650 |
R-factor | 0.20789 |
Rwork | 0.203 |
R-free | 0.30341 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1is5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.515 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.700 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.130 | 0.530 |
Number of reflections | 15419 | |
<I/σ(I)> | 5.8 | 1.6 |
Completeness [%] | 85.8 | 85.7 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.2 M CACL2, 0.1 M TRIS PH 8.5, 20% PEG4000 |