4AGK
Crystal structure of capsid protein (110-267) from Aura virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-11-25 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.661, 35.262, 49.578 |
Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
Resolution | 48.450 - 1.810 |
R-factor | 0.17898 |
Rwork | 0.176 |
R-free | 0.23151 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kxa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.567 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.270 |
Number of reflections | 11410 | |
<I/σ(I)> | 22.6 | 2.95 |
Completeness [%] | 90.2 | 42.8 |
Redundancy | 3.9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M BIS-TRIS AND POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000. |