4AGI
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.691, 79.847, 84.389 |
| Unit cell angles | 91.09, 88.89, 103.00 |
Refinement procedure
| Resolution | 84.220 - 1.600 |
| R-factor | 0.15363 |
| Rwork | 0.152 |
| R-free | 0.18508 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.270 |
| Number of reflections | 150144 | |
| <I/σ(I)> | 19.4 | 4.1 |
| Completeness [%] | 91.8 | 93.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM CACL2, 25% PEG 4K AND 100 MM TRIS, PH 8.5 METHYL-SE-FUCOSIDE (19 MM) |
