4AGG
Structure of a tetrameric galectin from Cinachyrella sp. (Ball sponge)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2010-03-18 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.005, 126.635, 80.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.220 - 2.980 |
R-factor | 0.19042 |
Rwork | 0.186 |
R-free | 0.27490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1is5 SIDECHAINS TRUNCATED |
RMSD bond length | 0.014 |
RMSD bond angle | 1.837 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.750 | 3.050 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.150 | 0.450 |
Number of reflections | 6308 | |
<I/σ(I)> | 11.7 | 2.1 |
Completeness [%] | 95.6 | 74.4 |
Redundancy | 8.7 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.2 M LITHIUM SULFATE, 0.1 M TRIS PH 8.5, 30% PEG3000 |