4AG6
Structure of VirB4 of Thermoanaerobacter pseudethanolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 109.286, 112.765, 156.579 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.956 - 2.350 |
| R-factor | 0.2282 |
| Rwork | 0.226 |
| R-free | 0.26580 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE (RESOLVE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 80446 | |
| <I/σ(I)> | 14.8 | 4.1 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 3350; 100 MM BIS-TRIS, PH 5.75; 200 MM AMMONIUM SULPHATE |
