4AG5

Structure of VirB4 of Thermoanaerobacter pseudethanolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-22
Detector	ADSC QUANTUM 315r
Spacegroup name	P 2 21 21
Unit cell lengths	108.080, 110.800, 156.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.040 - 2.450
R-factor	0.2362
Rwork	0.233
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ag6
RMSD bond length	0.009
RMSD bond angle	1.152
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.040	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.060	0.490
Number of reflections	69350
<I/σ(I)>	14.3	3
Completeness [%]	99.6	99.5
Redundancy	5.1	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1