4AFZ
Human Chymase - Fynomer Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.269, 58.341, 93.032 |
| Unit cell angles | 90.00, 103.76, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.250 |
| R-factor | 0.18107 |
| Rwork | 0.178 |
| R-free | 0.24076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN HOUSE STRUCTURE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.416 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 32077 | |
| <I/σ(I)> | 8.09 | 1.22 |
| Completeness [%] | 98.9 | 92.7 |
| Redundancy | 3.38 | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM SODIUM TARTRATE TETRAHYDRATE, 20% PEG 3350 |
