4AFD
Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens with a partially bound cellotetraose moeity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 48.773, 48.773, 193.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.240 - 1.340 |
| R-factor | 0.16655 |
| Rwork | 0.165 |
| R-free | 0.18681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aek |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.623 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.240 | 1.410 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 30670 | |
| <I/σ(I)> | 26.3 | 5 |
| Completeness [%] | 96.9 | 93.6 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 292 | 101 MG/ML PROTEIN COCRYSTALLISED WITH 1 0 MM CELLOHEXAOSE AT 292 K WITH 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH 4.6, 26% W/V PEG 2K MME. 30% GLYCEROL WAS USED AS CRYOPROTECTANT IN MOTHER LIQUOR SOLUTION. |
