4AFC
Crystal Structure of subtype-switched Epithelial Adhesin 1 to 6 A domain (Epa1to6A) from Candida glabrata in complex with Galb1-3Glc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.430, 103.890, 69.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.620 - 1.550 |
| R-factor | 0.14455 |
| Rwork | 0.144 |
| R-free | 0.17146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4af9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.620 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 39090 | |
| <I/σ(I)> | 26 | 3.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES PH 6 0.1 M AMMONIUM SULFATE 32.5% PEG 5000 MME 0.025 M LACTOSE |
