4AF7
PEA FNR C266M MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.735, 91.732, 139.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| R-factor | 0.24637 |
| Rwork | 0.243 |
| R-free | 0.29573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qg0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 3.000 |
| High resolution limit [Å] | 2.500 | 2.850 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 22623 | |
| <I/σ(I)> | 11.9 | 3.2 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 15% PEG1500, pH 8.0 |
