4AF5
Structure of the C. glutamicum AcnR Crystal Form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 34.640, 72.730, 73.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.200 - 1.900 |
| R-factor | 0.19646 |
| Rwork | 0.194 |
| R-free | 0.24632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ac6 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.956 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.310 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.660 |
| Number of reflections | 15067 | |
| <I/σ(I)> | 10 | 3.6 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 1 M AMMONIUM PHOSPHATE; 100 MM SODIUM CITRATE PH 4 |
