4AF1
Archeal Release Factor aRF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-30 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.020, 31.990, 111.150 |
| Unit cell angles | 90.00, 113.47, 90.00 |
Refinement procedure
| Resolution | 64.160 - 2.000 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dt9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.088 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.160 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 1.230 |
| Number of reflections | 29222 | |
| <I/σ(I)> | 13.4 | 2.3 |
| Completeness [%] | 94.7 | 48 |
| Redundancy | 8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | VAPOR DIFFUSION IN SITTING DROPS WITH RESERVOIR CONTAINING 1.8-2.2 M NA MALONATE (PH 8), 50 MM MES PH 6.5, 7 MM 2-MERCAPTOETHANOL. |
