4AEY
Crystal structure of FolX from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 133.980, 133.980, 133.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.990 - 3.000 |
| R-factor | 0.2843 |
| Rwork | 0.283 |
| R-free | 0.31070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b9l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.740 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 1.370 |
| Number of reflections | 4383 | |
| <I/σ(I)> | 34.3 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.7 | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40 % (V/V) 1,2-PROPANEDIOL, 100 MM HEPES PH 7.5 |
