4AEM
Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.320, 79.320, 44.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.690 - 2.100 |
| R-factor | 0.17353 |
| Rwork | 0.171 |
| R-free | 0.22704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aek |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.213 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.690 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 8165 | |
| <I/σ(I)> | 15.3 | 2.4 |
| Completeness [%] | 83.6 | 53.4 |
| Redundancy | 11.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.76 | 292 | 100 MG/ML PROTEIN WAS USED AT 292 K WITH 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH 4.6, 30% W/V PEG 2K MME. 30% GLYCEROL WAS USED AS CRYOPROTECTANT |
