4AEK
Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793, 0.97934, 0.9769 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 47.859, 47.859, 191.598 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.450 - 1.750 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.22712 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.020 |
RMSD bond angle | 2.076 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SAS (AUTORICKSAW) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.870 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.320 |
Number of reflections | 13971 | |
<I/σ(I)> | 10.4 | 1.7 |
Completeness [%] | 99.1 | 94.8 |
Redundancy | 5.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 292 | 0.23 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.6, 26%(M/V) PEG 2K MME GROWN AT 292 K, USED 30% GLYCEROL AS CRYOPROTECTANT |