4AEJ
Crystal structure of Human fibrillar procollagen type III C- propeptide trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.930, 89.270, 101.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 2.210 |
| R-factor | 0.20308 |
| Rwork | 0.201 |
| R-free | 0.23686 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.500 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.110 | 0.810 |
| Number of reflections | 38676 | |
| <I/σ(I)> | 19 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.2 M NACL |
