4AE9
Structure and function of the Human Sperm-Specific Isoform of Protein Kinase A (PKA) Catalytic Subunit C alpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.396, 89.971, 115.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.020 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.799 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.020 | 7.270 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.230 |
| Number of reflections | 34845 | |
| <I/σ(I)> | 9.7 | 4.5 |
| Completeness [%] | 97.0 | 98.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % PEG 3350, 0.2 M POTASSIUM ACETATE, 5 % POLYPROPYLENE GLYCOL P400 |
