4AE6
Structure and Function of the Human Sperm-Specific Isoform of Protein Kinase A (PKA) Catalytic Subunit Calpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.776, 89.495, 114.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.240 - 2.100 |
| R-factor | 0.21864 |
| Rwork | 0.215 |
| R-free | 0.28328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smh |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.871 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.440 | 6.640 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 46218 | |
| <I/σ(I)> | 14.9 | 3.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.83 | 5.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % PEG 3350, 0.2 M POTASSIUM ACETATE, 5 % POLYPROPYLENE GLYCOL P400 |
