4AE3
Crystal structure of ammosamide 272:myosin-2 motor domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-06 |
| Detector | BRUKER |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.050, 145.520, 153.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.914 - 2.500 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yv3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.554 |
| Data reduction software | PROTEUM2 |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.910 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.570 | |
| Number of reflections | 34133 | |
| <I/σ(I)> | 16.68 | 2.16 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 76.61 | 49.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM HEPES PH 7.6, 140 MM NACL, 11% W/V PEG5K-MME, 2% (V/V) MPD |
