4ADZ
Crystal Structure of the apo form of a Copper-sensitive operon Regulator (CsoR) protein from Streptomyces lividans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-24 |
Detector | ADSC CCD |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 41.630, 54.550, 91.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.550 - 1.700 |
R-factor | 0.18754 |
Rwork | 0.185 |
R-free | 0.22858 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.663 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.900 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.058 | 0.280 |
Number of reflections | 22220 | |
<I/σ(I)> | 12.1 | 2.1 |
Completeness [%] | 93.8 | 71.7 |
Redundancy | 3.1 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 1.26 M AMMONIUM SULPHATE, 0.1 M SODIUM CITRATE PH 4 |