4ADY

Crystal structure of 26S proteasome subunit Rpn2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-20
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	73.960, 148.660, 114.030
Unit cell angles	90.00, 107.39, 90.00

Refinement procedure

Resolution	70.579 - 2.700
R-factor	0.1839
Rwork	0.183
R-free	0.24020
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.192
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.330	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.600
Number of reflections	64456
<I/σ(I)>	9.1	2.4
Completeness [%]	99.2	96.4
Redundancy	5.2	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	CRYSTALS WERE GROWN BY VAPOUR DIFFUSION IN HANGING DROPS AT 18 C. PROTEIN AT A CONCENTRATION OF 18 MG/ML WAS MIXED WITH AN EQUAL VOLUME OF RESERVOIR SOLUTION CONTAINING 100 MM BIS-TRIS PROPANE PH 7.0, 200 MM POTASSIUM SODIUM TARTRATE AND 16-18% (W/V) PEG 3350.