4ADV
Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13.5 |
| Software | UCSF Chimera,IMAGIC,SPIDER |
| Symmetry | C1 |
