4ADS
Crystal structure of plasmodial PLP synthase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 160.008, 160.008, 583.243 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.610 |
| R-factor | 0.29442 |
| Rwork | 0.293 |
| R-free | 0.31374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.234 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.610 | 3.600 |
| Rmerge | 0.140 | 0.380 |
| Number of reflections | 47666 | |
| <I/σ(I)> | 6.41 | 1.7 |
| Completeness [%] | 96.8 | 64.5 |
| Redundancy | 5.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 0.1 M NA/K PHOSPHATE-PHOSPHATE PH 6.2, 0.2 M NACL, 6% PEG 8000, 0.01 M L-GLUTAMINE |
