4ADI
Crystal structure of the Rubella virus envelope glycoprotein E1 in post-fusion form (crystal form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2007-11-02 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 129.980, 121.380, 126.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.1682 |
Rwork | 0.167 |
R-free | 0.18310 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.300 | 1.870 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.090 | 0.500 |
Number of reflections | 188417 | |
<I/σ(I)> | 16.7 | 2 |
Completeness [%] | 98.3 | 88.1 |
Redundancy | 8.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1-3% PEG 4K, 0.1M NAHEPES PH7.5-8, 30% GLYCEROL |