4ADB
Structural and functional study of succinyl-ornithine transaminase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.708, 118.428, 109.792 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 109.050 - 2.200 |
| R-factor | 0.17323 |
| Rwork | 0.171 |
| R-free | 0.21885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pb2 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.076 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.610 |
| Number of reflections | 118526 | |
| <I/σ(I)> | 14.6 | 3.4 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.17 M SODIUM MALONATE PH 7.0, 0.09 M TRIS CHLORIDE PH 8.0, 0.02 M DI-ETHYLAMMONIUM FORMATE IN 150 PLUS 150 NL DROPS. |
