4AD9
Crystal structure of human LACTB2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.470, 95.690, 135.760 |
| Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
| Resolution | 46.850 - 2.600 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.22370 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.580 |
| Rmerge | 0.070 | 0.070 |
| Number of reflections | 77582 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 10.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.20M NABR, 0.1 M BTPROP PH 6.5, 20.0% PEG 3350, 10.0% ETHYLENE GLYCOL |
