4AD1
Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2011-05-30 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 108.540, 108.540, 67.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.370 - 1.900 |
R-factor | 0.15132 |
Rwork | 0.150 |
R-free | 0.18549 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PREVIOUSLY SOLVED STRUCTURE OF CLOSELY RELATED HOMOLOG |
RMSD bond length | 0.012 |
RMSD bond angle | 1.205 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.046 | 0.150 |
Number of reflections | 31100 | |
<I/σ(I)> | 24.2 | 8.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1 M SODIUM ACETATE PH 4.9, 20% W/V PEG 2000 MME, 1.6% PGA-LM (POLY-GAMMA-GLUTAMIC ACID LOW MOLECULAR WEIGHT) |