4ACV
Listeria monocytogenes Antigen B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-01 |
| Detector | ADSC CCD |
| Wavelength(s) | 1.0332, 0.9792 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 122.456, 122.456, 122.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.724 - 2.401 |
| R-factor | 0.1878 |
| Rwork | 0.185 |
| R-free | 0.23520 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.590 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 12093 | |
| <I/σ(I)> | 30.6 | 8.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.7 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 4M NA FORMATE, pH 8.0 |
