4ACI
Structure of the C. glutamicum AcnR Crystal Form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.210, 72.790, 145.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.790 - 1.650 |
| R-factor | 0.14466 |
| Rwork | 0.142 |
| R-free | 0.20488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ac6 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.620 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.780 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 44662 | |
| <I/σ(I)> | 9.4 | 2.7 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 1M AMMONIUM PHOSPHATE; 100MM NACITRATE PH4 |
