4AC8
R2-like ligand binding Mn-Fe oxidase from M. tuberculosis with an organized C-terminal helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.990, 210.850, 139.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.720 - 2.750 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ee4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.720 | 2.830 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.140 | 0.610 |
| Number of reflections | 39247 | |
| <I/σ(I)> | 9.75 | 2.83 |
| Completeness [%] | 92.1 | 94.4 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
