4AC1
The structure of a fungal endo-beta-N-acetylglucosaminidase from glycosyl hydrolase family 18, at 1.3A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Collection date | 2008-04-09 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.438, 63.874, 59.360 |
| Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.18473 |
| Rwork | 0.184 |
| R-free | 0.20179 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.420 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 63365 | |
| <I/σ(I)> | 12.3 | 5.5 |
| Completeness [%] | 99.2 | 95.6 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | AS DESCRIBED IN PUBLICATION, pH 5 |
