4ABX

Crystal structure of Deinococcus radiodurans RecN coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-01
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	73.210, 43.970, 133.630
Unit cell angles	90.00, 97.71, 90.00

Refinement procedure

Resolution	45.933 - 2.041
R-factor	0.2033
Rwork	0.201
R-free	0.25020
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.500	2.150
High resolution limit [Å]	2.040	2.040
Rmerge	0.070	0.320
Number of reflections	52532
<I/σ(I)>	8.4	2.8
Completeness [%]	97.1	98.2
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M HEPES PH 7, 30% PEG 6000, 1 M LICL 3% 1,2,3-HEPTANETRIOL.