4ABT
Crystal structure of Type IIF restriction endonuclease NgoMIV with cognate uncleaved DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.899, 90.081, 92.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.950 - 2.220 |
| R-factor | 0.18543 |
| Rwork | 0.180 |
| R-free | 0.23483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fiu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.815 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.950 | 2.270 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 28869 | |
| <I/σ(I)> | 8 | 1.8 |
| Completeness [%] | 98.2 | 84.1 |
| Redundancy | 4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | NAHEPES 0.1M, CACL2 0.2M, MPD 24%, PH 7.5 |
