4ABT
Crystal structure of Type IIF restriction endonuclease NgoMIV with cognate uncleaved DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-03 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.899, 90.081, 92.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.950 - 2.220 |
R-factor | 0.18543 |
Rwork | 0.180 |
R-free | 0.23483 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fiu |
RMSD bond length | 0.019 |
RMSD bond angle | 1.815 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.950 | 2.270 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.080 | 0.340 |
Number of reflections | 28869 | |
<I/σ(I)> | 8 | 1.8 |
Completeness [%] | 98.2 | 84.1 |
Redundancy | 4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | NAHEPES 0.1M, CACL2 0.2M, MPD 24%, PH 7.5 |