4ABL
HUMAN PARP14 (ARTD8, BAL2) - MACRO DOMAIN 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.705, 41.066, 108.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.790 - 1.150 |
| R-factor | 0.16222 |
| Rwork | 0.161 |
| R-free | 0.19132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4abk |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.589 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 53461 | |
| <I/σ(I)> | 20.9 | 3.9 |
| Completeness [%] | 97.8 | 82.7 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEGMME2000, 0.05M POTASSIUM BROMIDE |
