4AAY
Crystal Structure of the arsenite oxidase protein complex from Rhizobium species strain NT-26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2011-07-11 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 149.130, 232.960, 141.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 141.870 - 2.700 |
R-factor | 0.19433 |
Rwork | 0.193 |
R-free | 0.21128 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g8j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.393 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER (FOR MR) |
Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 0.370 |
Number of reflections | 135790 | |
<I/σ(I)> | 18 | 6.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES SODIUM PH 7.5, 2% PEG 400, 2.0 M AMMONIUM SULPHATE, 10% GLYCEROL |