4A9A
Structure of Rbg1 in complex with Tma46 dfrp domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.200, 224.890, 84.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.220 - 2.670 |
| R-factor | 0.19655 |
| Rwork | 0.196 |
| R-free | 0.22212 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.420 | 2.820 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 47671 | |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.14 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.38 SODIUM FORMATE, 0.2-0.25 SODIUM CITRATE PH 6.5 |
