4A8Z

Human myelin P2 protein, K112Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-19
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	65.710, 65.710, 101.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.608 - 1.801
R-factor	0.179
Rwork	0.178
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wut
RMSD bond length	0.012
RMSD bond angle	1.368
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.060	1.270
Number of reflections	21058
<I/σ(I)>	30.2	2
Completeness [%]	99.4	95.6
Redundancy	11.8	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1