4A8Z
Human myelin P2 protein, K112Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-19 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.710, 65.710, 101.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.608 - 1.801 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wut |
RMSD bond length | 0.012 |
RMSD bond angle | 1.368 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 1.270 |
Number of reflections | 21058 | |
<I/σ(I)> | 30.2 | 2 |
Completeness [%] | 99.4 | 95.6 |
Redundancy | 11.8 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |