4A8Y
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.760, 89.290, 140.990 |
Unit cell angles | 90.00, 101.88, 90.00 |
Refinement procedure
Resolution | 67.610 - 3.410 |
R-factor | 0.2196 |
Rwork | 0.218 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hhs |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 68.000 |
High resolution limit [Å] | 3.400 |
Rmerge | 0.170 |
Number of reflections | 30875 |
<I/σ(I)> | 10 |
Completeness [%] | 87.0 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |