4A8X

Structure of the core ASAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-11
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	38.868, 73.331, 50.754
Unit cell angles	90.00, 110.99, 90.00

Refinement procedure

Resolution	36.289 - 1.900
R-factor	0.1877
Rwork	0.186
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1RK8 AND 4A6Q
RMSD bond length	0.012
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.570	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.400
Number of reflections	25760
<I/σ(I)>	14.4	3.8
Completeness [%]	98.9	100
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		100 MM TRIS PH 7.5 25% PEG 3350