4A8W
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.910, 91.430, 141.570 |
Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
Resolution | 44.510 - 3.040 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hhs |
RMSD bond length | 0.008 |
RMSD bond angle | 1.030 |
Refinement software | BUSTER-TNT (BUSTER 2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.000 | |
High resolution limit [Å] | 3.000 | |
Rmerge | 0.180 | |
Number of reflections | 51249 | |
<I/σ(I)> | 14.7 | 2.4 |
Completeness [%] | 99.0 | 98 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12% PEG 20000, 100 MM MES (PH 6.5) |