4A8Q
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.440, 92.850, 141.230 |
Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
Resolution | 77.070 - 3.060 |
R-factor | 0.2056 |
Rwork | 0.205 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 77.200 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.180 |
Number of reflections | 50754 |
<I/σ(I)> | 13 |
Completeness [%] | 98.0 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12% PEG 20000, 100 MM MES (PH 6.5) |