4A7P

Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-02
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	109.030, 109.030, 175.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 3.403
Rwork	0.230
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	1.011
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.080	0.730
Number of reflections	27657
<I/σ(I)>	25.1	3.8
Completeness [%]	99.2	99.5
Redundancy	13.1	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	1 ML DROPS OF PROTEIN SOLUTION, 5.5 MG/ML SE-MET UGDG IN 25 MM TRIS-HCL PH 8.3, 50 MM NACL, 2.5 MM DTT AND 1 MM NAD, WERE MIXED WITH 1 ML DROPS OF PRECIPITANT SOLUTION, 0.1 M MES PH 6.5, 40 % (W/V) PEG 200, AND LET EQUILIBRATE BY VAPOR DIFFUSION AGAINST 500 ML PRECIPITANT SOLUTION AT 293 K.