4A75
The Lin28b Cold shock domain in complex with hexathymidine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY |
Synchrotron site | BESSY |
Beamline | BL14-1 |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.799, 81.822, 99.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.910 - 1.750 |
R-factor | 0.17239 |
Rwork | 0.171 |
R-free | 0.19801 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.476 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.910 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.060 | |
Rmeas | 0.078 | 0.390 |
Number of reflections | 39188 | |
<I/σ(I)> | 13.2 | 3.3 |
Completeness [%] | 89.4 | 87.8 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 0.75 mM protein-DNA complex (in 20 mM Tris/HCl, pH 7.5, 100 mM KCl, 2 mM MgCl2 and 1 mM DTT) and 0.1 mM Bis-Tris, pH 5.5, 15% (w/v) PEG 3350 and 0.1 M sodium thiocyanate as reservoir buffer |