4A72
Crystal structure of the omega transaminase from Chromobacterium violaceum in a mixture of apo and PLP-bound states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.350, 62.120, 119.240 |
| Unit cell angles | 105.27, 90.66, 104.43 |
Refinement procedure
| Resolution | 26.391 - 2.400 |
| R-factor | 0.1763 |
| Rwork | 0.173 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6r |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 61839 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 95.5 | 82.6 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 200 NL OF PROTEIN AT 12 MG/ML IN 1 MM CYCLOSERINE MIXED WITH 200 NL OF RESERVOIR CONSISTING OF 0.1 M HEPES PH 7.5, 150-300 MM NACL AND 22.5-27.5% PEG 4000 |
