4A6X
RadA C-terminal ATPase domain from Pyrococcus furiosus bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.320, 87.350, 61.880 |
| Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
| Resolution | 43.675 - 1.548 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE ENTRY 4A6P |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.115 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 59656 | |
| <I/σ(I)> | 11.5 | 1.9 |
| Completeness [%] | 95.7 | 93.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 60MM NA2HPO4 PH 6.0; 15% PEG1000 |
