4A66
Mutations in the neighbourhood of CotA-laccase trinuclear site: D116A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRUKER PT135 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.683, 101.683, 136.611 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 1.950 |
| R-factor | 0.17554 |
| Rwork | 0.174 |
| R-free | 0.20306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.428 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.680 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.290 |
| Number of reflections | 60037 | |
| <I/σ(I)> | 17.5 | 3.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 35% ETHYLENE GLYCOL, pH 7.6 |
