4A62
ParM from R1 plasmid in complex with peptide from C-terminus of ParR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-09 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 146.850, 146.850, 171.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.615 - 2.200 |
| R-factor | 0.2043 |
| Rwork | 0.201 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mwm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.040 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 36167 | |
| <I/σ(I)> | 20 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M CITRIC ACID PH 5.0, 2.4 M AMMONIUM SULPHATE |
