4A61

ParM from plasmid R1 in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-23
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 2 2
Unit cell lengths	63.541, 63.541, 164.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.335 - 2.000
R-factor	0.2114
Rwork	0.209
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mwm
RMSD bond length	0.005
RMSD bond angle	0.936
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	PHENIX ((PHENIX.REFINE: DEV_874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.240	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.090	0.510
Number of reflections	23264
<I/σ(I)>	12.1	2.3
Completeness [%]	98.4	90.9
Redundancy	6.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.6		20% PEG 6000, 100 MM BICINE PH 8.6, 1M LICL